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Functional analysis of the Pseudomonas syringae rulAB determinant in tolerance to ultraviolet B (290–320 nm) radiation and distribution of rulAB among P. syringae pathovars
Author(s) -
Sundin George W.,
Murillo Jesús
Publication year - 1999
Publication title -
environmental microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.954
H-Index - 188
eISSN - 1462-2920
pISSN - 1462-2912
DOI - 10.1046/j.1462-2920.1999.00008.x
Subject(s) - pseudomonas syringae , biology , plasmid , microbiology and biotechnology , replicon , pseudomonadaceae , pseudomonas , pathogen , pseudomonadales , bacteria , gene , genetics
The effect of the plasmid‐encoded rulAB (resistance to ultraviolet radiation) determinant on responses of Pseudomonas syringae to ultraviolet‐B (UV‐B) radiation and the distribution of rulAB among pathovars of P. syringae were determined. The cloned rulAB determinant and the native rulAB + plasmid pPSR1 both conferred approximately a 10‐fold increase in survival on P. syringae pv. syringae FF5 following increasing doses of UV‐B radiation. rulAB + P. syringae strains also maintained significantly larger epiphytic populations on leaf surfaces irradiated with UV‐B. rulAB ‐insertional mutants, constructed in two native rulAB + strains, were from 10‐ to 100‐fold more sensitive to UV‐B radiation. The UV tolerance phenotype and the rulAB genes were widely distributed among P. syringae pathovars isolated from varied plant hosts throughout the world and within a broad range of genotypic backgrounds of P. syringae pv. syringae. With one exception, the rulAB determinant was harboured on pPT23A‐like plasmids; these replicons are indigenous residents of the species P. syringae and also tend to encode determinants of importance in host–pathogen interactions.