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Detection of Rhopalosiphum insertum (apple‐grass aphid) predation by the predatory mite Anystis baccarum using molecular gut analysis
Author(s) -
Cuthbertson Andrew G. S.,
Fleming Colin C.,
Murchie Archie K.
Publication year - 2003
Publication title -
agricultural and forest entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.755
H-Index - 55
eISSN - 1461-9563
pISSN - 1461-9555
DOI - 10.1046/j.1461-9563.2003.00181.x
Subject(s) - macrosiphum euphorbiae , biology , polymerase chain reaction , myzus persicae , aphid , mitochondrial dna , microbiology and biotechnology , polymerase , dna , aphididae , genetics , gene , botany , pest analysis , homoptera
1 A simple, yet sensitive polymerase chain reaction based technique was developed for the detection of the apple‐grass aphid Rhopalosiphum insertum in the gut of Anystis baccarum , a predatory mite. 2 A range of conserved polymerase chain reaction primers for insect mitochondrial and ribosomal DNA were tested in order to amplify R. insertum DNA. The mitochondrial DNA primers LrRNAR2 + N1F1, amplified a region between the ND1 and large subunit RNA genes. 3 DNA sequencing of the R. insertum ND1‐LRNA polymerase chain reaction product allowed aphid‐specific polymerase chain reaction primers to be designed. These amplified a 283‐bp product from individual aphids. No polymerase chain reaction product was amplified from individual A. baccarum . 4 Using the aphid‐specific primers against A. baccarum fed on R. insertum , the diagnostic 283‐bp product was amplified. 5 Two restriction enzymes ( Rsa I and Alu I) produced patterns that allowed unambiguous identification of R. insertum DNA from that of Macrosiphum euphorbiae and Myzus persicae .