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Mortality of second‐instar larvae of Tuta absoluta produced by the hexane extract of Lycopersicon hirsutum f. glabratum (PI 134417) leaves
Author(s) -
Verçosa de Magalhães Sergio Tinôco,
Jham Gulab N.,
Picanço Marcelo C.,
Magalhães Gouvan
Publication year - 2001
Publication title -
agricultural and forest entomology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.755
H-Index - 55
eISSN - 1461-9563
pISSN - 1461-9555
DOI - 10.1046/j.1461-9555.2001.00116.x
Subject(s) - instar , lycopersicon , tuta absoluta , biology , larva , hexane , petri dish , horticulture , botany , chromatography , chemistry , genetics , gelechiidae
1 The mortality resulting from exposure of second‐instar larvae of Tuta absoluta to a hexane extract of Lycopersicon hirsutum f. glabratum (PI 134417) leaves was evaluated in the laboratory. 2 A crude hexane extract was fractionated on a silica gel column to produce 17 fractions. The toxicity of the fractions was evaluated on T. absoluta larvae at 2 and/or 6 mg/10 larvae/Petri dish along with a control. 3 Only two fractions, 4.50 and 10.18% of the total hexane extract, were found to result in very high mortality of second‐instar T. absoluta . 4 All the 17 fractions were analysed by capillary gas chromatography/mass spectrometry (GC/MS). The two most toxic fractions were virtually identical and contained mainly undeca‐2‐one (2‐UD) and trideca‐2‐one (2‐TD). 5 A third major sub‐fraction 13 (50.73% of the total hexane extract) was further fractionated on a silica gel column to obtain five additional sub‐fractions. The toxicity of all the five sub‐fractions was evaluated using second‐instar T. absoluta at 2 mg/10 larvae/Petri dish and also analysed by GC/MS. The most toxic sub‐fractions contained mostly 2‐UD and 2‐TD. 6 The results suggest that 2‐UD and 2‐TD could be related to resistance of L. hirsutum to T. absoluta .