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Distinct receptors and different transduction mechanisms for ATP and adenosine at the frog motor nerve endings
Author(s) -
Sokolova E.,
Grishin S.,
Shakirzyanova A.,
Talantova M.,
Giniatullin R.
Publication year - 2003
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.2003.02835.x
Subject(s) - ppads , p2y receptor , p2 receptor , receptor , agonist , suramin , biology , metabotropic receptor , purinergic receptor , microbiology and biotechnology , chemistry , biochemistry
Abstract Corelease of ATP with ACh from motor endings suggests a physiological role for ATP in synaptic transmission. We previously showed that, on skeletal muscle, ATP directly inhibited ACh release via presynaptic P2 receptors. The receptor identification (P2X or P2Y) and its transduction mechanism remained, however, unknown. In the present study using the voltage‐clamp technique we analyzed the properties of presynaptic ATP receptors and subsequent effector mechanisms. ATP or adenosine presynaptically depressed multiquantal end‐plate currents, with longer latency for ATP action. ATPγS, agonist at P2X receptors, or Bz‐ATP, agonist at P2X 7 receptors, were ineffective. The action of ATP was prevented by suramin and unchanged by PPADS or TNP‐ATP, antagonists of P2X receptors, or RB‐2, a blocker of certain P2Y receptors. The depressant action of ATP was reproduced by UTP, metabotropic P2Y receptor agonist. Pertussis toxin (PTX), antagonist of G i/o ‐proteins, and inhibitors of phosphatidylcholine specific PLC (D609) and PKC (staurosporine or chelerythrine) prevented the effect of ATP while blockers of PLA 2 (OBAA) and COX (aspirin or indomethacin) attenuated it. Inhibitors of phosphatidylinositide‐specific PLC (U73122), guanylylcyclase (ODQ), PKA (Rp‐cAMPS) or PLD (1‐butanol) did not affect the action of ATP. No inhibitor of second messengers (except PTX) changed the action of adenosine. Our data indicate, for motor nerve endings, the existence of inhibitory P2Y receptors coupled to multiple intracellular cascades including phosphatidylinositide‐specific PLC/PKC/PLA 2 /COX. This divergent presynaptic P2 signalling (unlike the single effector mechanism for P1 receptors) could provide feedback inhibition of transmitter release and perhaps be involved in presynaptic plasticity.