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Functional characterization of a neuropeptide F‐like receptor from Drosophila melanogaster
Author(s) -
Feng Guoping,
Reale Vincenzina,
Chatwin Heather,
Kennedy Karen,
Venard Renée,
Ericsson Christer,
Yu Kweon,
Evans Peter D.,
Hall Linda M.
Publication year - 2003
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.2003.02719.x
Subject(s) - biology , drosophila melanogaster , g protein coupled receptor , neuropeptide , 5 ht5a receptor , receptor , genetics , microbiology and biotechnology , gene
A cDNA clone encoding a seven‐transmembrane domain, G‐protein‐coupled receptor (NPFR76F, also called GPCR60), has been isolated from Drosophila melanogaster . Deletion mapping showed that the gene encoding this receptor is located on the left arm of the third chromosome at position 76F. Northern blotting and whole mount in situ hybridization have shown that this receptor is expressed in a limited number of neurons in the central and peripheral nervous systems of embryos and adults. Analysis of the deduced amino acid sequence suggests that this receptor is related to vertebrate neuropeptide Y receptors. This Drosophila receptor shows 62–66% similarity and 32–34% identity to type 2 neuropeptide Y receptors cloned from a variety of vertebrate sources. Coexpression in Xenopus oocytes of NPFR76F with the promiscuous G‐protein G α16 showed that this receptor is activated by the vertebrate neuropeptide Y family to produce inward currents due to the activation of an endogenous oocyte calcium‐dependent chloride current. Maximum receptor activation was achieved with short, putative Drosophila neuropeptide F peptides (Drm‐sNPF‐1, 2 and 2s). Neuropeptide F‐like peptides in Drosophila have been implicated in a signalling system that modulates food response and social behaviour. The identification of this neuropeptide F‐like receptor and its endogenous ligand by reverse pharmacology will facilitate genetic and behavioural studies of neuropeptide functions in Drosophila .