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Purinergic receptors on microglial cells: functional expression in acute brain slices and modulation of microglial activation in vitro
Author(s) -
Boucsein Clemens,
Zacharias Robert,
Färber Katrin,
Pavlovic Sanja,
Hanisch UweKarsten,
Kettenmann Helmut
Publication year - 2003
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.2003.02663.x
Subject(s) - purinergic receptor , microglia , microbiology and biotechnology , astrocyte , receptor , neuroglia , purinergic signalling , extracellular , biology , chemistry , neuroscience , inflammation , biochemistry , immunology , central nervous system , adenosine receptor , agonist
Microglial cells are the pathologic sensors in the brain. ATP released from damaged cells is a candidate for signalling neural injury to microglia. Moreover, ATP is an extracellular messenger for propagating astrocyte activity in the form of Ca 2+ waves. To test for the functional expression of purinoreceptors in microglial cells we employed the patch‐clamp technique in acute slices of adult mouse brain. ATP triggered a nonselective cationic and a K + current. Pharmacological screening with purinergic ligands indicated the presence of P2Y1 and P2Y2/4 receptors linked to the activation of a K + current and P2X receptors, including P2X7, linked to the activation of a nonselective cationic current. These findings suggest that microglial cells in situ express different purinergic receptors with distinct sensitivity and functional coupling. To test for the involvement of purinoreceptors in microglial activation, we stimulated cultured microglial cells with lipopolysaccharide and measured the release of tumour necrosis factor α, interleukin‐6, interleukin‐12 and macrophage inflammatory protein 1α, induction of K + outward currents and nitric oxide release. All these parameters were reduced in the presence of purinergic ligands, indicating that purinergic receptor activation attenuated indicators of microglial activation.