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Mitochondrial oxidative metabolism in motor neuron degeneration ( mnd ) mouse central nervous system
Author(s) -
Bertamini M.,
Marzani B.,
Guarneri R.,
Guarneri P.,
Bigini P.,
Mennini T.,
Curti D.
Publication year - 2002
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.2002.02299.x
Subject(s) - tbars , spinal cord , lumbar spinal cord , central nervous system , lumbar , citrate synthase , medicine , endocrinology , motor neuron , oxidative stress , neuroscience , biology , lipid peroxidation , anatomy , biochemistry , enzyme
The mnd mouse spontaneously develops slowly evolving motoneuron pathology leading to progressive motor impairment. There is strong evidence that a complex interplay between oxidative stress, mitochondria abnormalities and alteration of glutamate neurotransmission plays an important role in the pathogenesis of motor neuron diseases. Therefore, we investigated the presence of mitochondrial dysfunction in frontal, central (comprising the motor area) and occipital regions of the cerebral cortex and in the spinal cord of 35‐week‐old mnd mice. Lipid peroxide derivatives reacting with thiobarbituric acid (TBARS) were measured in the cervical, thoracic and lumbar spinal cord. In addition biochemical and behavioural analyses were carried out in mnd mice chronically treated with l ‐carnitine from the 11th to the 34th week of life ( mnd T mice). Slight but significant alterations of mitochondrial enzyme activities were seen in the mnd cortical regions. The central area was the most affected and both complex I, IV and citrate synthase were decreased with respect to controls. The rate of oxygen consumption (QO 2 ) was markedly decreased in both the upper (cervical + upper portion of the thoracic region) and lower (lumbar + lower portion of the thoracic region) mnd spinal cord. The level of TBARS showed a rostro‐caudal trend to increase, being 30% higher in the lumbar tract of mnd mice in comparison with controls. l ‐carnitine treatment increased the mitochondrial enzyme activities in cortical regions towards control value and was effective in enhancing QO 2 and decreasing TBARS levels in the spinal cord of mnd T. Behavioural testing showed that l ‐carnitine significantly delayed the onset of motor behaviour impairment. This beneficial effect was declining at 35 week of age, when the biochemical measurements were performed.