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Clustering of δ glutamate receptors is regulated by the actin cytoskeleton in the dendritic spines of cultured rat Purkinje cells
Author(s) -
Hirai Hirokazu
Publication year - 2000
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.2000.00938.x
Subject(s) - dendritic spine , microbiology and biotechnology , actin remodeling of neurons , glutamate receptor , actin cytoskeleton , biology , cytoskeleton , postsynaptic density , postsynaptic potential , cytochalasin d , dendritic filopodia , neurotransmitter receptor , long term depression , receptor , neuroscience , ampa receptor , cell , hippocampal formation , biochemistry
The interaction of neurotransmitter receptors with the cytoskeleton is an important mechanism for the targeting of receptors to the postsynaptic membrane. Using cytoskeleton‐perturbing agents, it was demonstrated that δ glutamate receptors, predominantly expressed on the dendritic spines of cerebellar Purkinje cells, are anchored to the actin cytoskeleton. The number of δ glutamate receptor‐immunoreactive clusters was dramatically decreased following treatment of the Purkinje cells with the actin‐disrupting agents, cytochalasin D or latrunculin A, without any significant effect on the number of presynaptic contacts of the granule cell axons. The clusters disrupted by latrunculin A were re‐established 24 h after removal of the drug. These results suggest that morphological changes in the actin cytoskeleton regulate the δ glutamate receptor clustering on the dendritic spines, and may affect synaptic efficacy and plasticity.