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Lack of glucocorticoids attenuates the self‐stimulation‐induced increase in the in vivo synthesis rate of dopamine but not serotonin in the rat nucleus accumbens
Author(s) -
Nakahara Daiichiro,
Nakamura Masato,
Oki Yutaka,
Ishida Yasushi
Publication year - 2000
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.2000.00031.x
Subject(s) - microdialysis , medial forebrain bundle , nucleus accumbens , endocrinology , stimulation , medicine , dopamine , chemistry , corticosterone , serotonin , in vivo , adrenalectomy , glucocorticoid , hormone , striatum , biology , receptor , microbiology and biotechnology
Our previous study demonstrated that intracranial self‐stimulation of the medial forebrain bundle can increase the in vivo synthesis turnover rate of dopamine (DA) and serotonin (5‐HT) in the nucleus accumbens of adrenal‐intact rats. The present study examined using microdialysis whether such increases in DA and 5‐HT syntheses are influenced by adrenal hormones, which are also activated following intracranial self‐stimulation. A decarboxylase inhibitor, NSD‐1015, was perfused through reversed microdialysis which enabled the simultaneous measurement of 3,4‐dihydroxyphenylalanine (DOPA) and 5‐hydroxytryptophan (5‐HTP) as an index of the in vivo turnover rate of DA and 5‐HT syntheses. Adrenalectomy (ADX) attenuated significantly the self‐stimulation‐induced increase in dialysate levels of DOPA but not 5‐HTP. Corticosterone (Cort) replacement reversed the attenuation in DOPA levels in adrenalectomized rats. The finding indicates that activation of DA synthesis in vivo in the nucleus accumbens during intracranial self‐stimulation is dependent on, whereas that of 5‐HT synthesis is independent of glucocorticoid modulation.