Premium
The intrinsic specification of γ‐aminobutyric acid type A receptor α6 subunit gene expression in cerebellar granule cells
Author(s) -
Bahn Sabine,
Wisden William,
Dunnett Stephen B.,
Svendsen Clive
Publication year - 1999
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.1999.00662.x
Subject(s) - aminobutyric acid , protein subunit , microbiology and biotechnology , chemistry , granule cell , cerebellum , granule (geology) , gene expression , neuroscience , biophysics , receptor , gene , biology , biochemistry , hippocampal formation , paleontology , dentate gyrus
The patterns of γ‐aminobutyric acid type A (GABA A ) receptor subunit gene expression in the brain are complex. For example, mouse hippocampal dentate granule cells express many subunit genes, whereas adult cerebellar granule cells, which may share differentiation mechanisms, have a smaller compliment and uniquely express the α6 subunit gene. To see how the α6 expression component arises, i.e. if intrinsically or environmentally specified, we used a mouse line (Δα6lacZ) with a β‐galactosidase reporter inserted into the α6 gene. Precursor cells from postnatal day 1 Δα6lacZ cerebellum were transplanted to the adult hippocampus and cerebellum of wild‐type mice; 4 weeks after transplantation, Δα6lacZ cells expressed α6‐lacZ in the hippocampus, amygdala and cerebellum. Thus, different adult environments support both the development and maintenance of α6 gene expression from cerebellar granule cell precursors. Establishing α6 gene expression is not likely to require specific patterns of neurotransmitter innervation or other factors present only in the developing brain; instead, α6 expression can be timed and maintained autonomously.