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Neuropeptide Y increases intracellular calcium in rat pinealocytes
Author(s) -
Simonneaux Valérie,
Rodeau JeanLuc,
Calgari Christiane,
Pévet Paul
Publication year - 1999
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.1999.00499.x
Subject(s) - pinealocyte , calcium in biology , intracellular , calcium , neuropeptide , neuropeptide y receptor , chemistry , endocrinology , microbiology and biotechnology , neuroscience , biology , medicine , melatonin , pineal gland , receptor
The pineal gland is mainly innervated by sympathetic fibres containing noradrenaline (NA) and neuropeptide Y (NPY). NA released at night stimulates melatonin synthesis via a β 1 ‐adrenergic‐induced increase in cyclic adenosine monophosphate (cAMP) concentration potentiated by an α 1 ‐adrenergic‐induced increase in Ca 2+ concentration. We previously showed that NPY acted on presynaptic Y2 receptors inhibiting NA release and on postsynaptic Y1 receptors stimulating melatonin synthesis. Here we used Fura‐2 imaging to assess the effect of NPY on the intracellular Ca 2+ concentration, [Ca 2+ ] i , in cultured rat pineal cells. In 84% of cells, on average, 10 n m NPY induced a progressive rise of [Ca 2+ ] i from its basal value of 102 ± 3 n m to a plateau of 180 ± 6 n m ( n = 467 cells), which lasted the time of NPY application. This effect of NPY appeared dependent on extracellular Ca 2+ .