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Fast confocal imaging of calcium released from stores in dendritic spines
Author(s) -
Korkotian Eduard,
Segal Menahem
Publication year - 1998
Publication title -
european journal of neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.346
H-Index - 206
eISSN - 1460-9568
pISSN - 0953-816X
DOI - 10.1046/j.1460-9568.1998.00219.x
Subject(s) - dendritic spine , ryanodine receptor , calcium , dendritic filopodia , calcium imaging , dendrite (mathematics) , hippocampal formation , calcium signaling , biology , thapsigargin , neuroscience , microbiology and biotechnology , chemistry , geometry , mathematics , organic chemistry
The emerging significance of calcium stores in neuronal plasticity and the assumed involvement of dendritic spines in long‐term plastic properties of neurons have led us to examine the presence and possible regulation of calcium stores in dendritic spines. Immunohistochemical staining for ryanodine receptors was found in dendritic spines of cultured hippocampal neurons. Confocal microscopic imaging of calcium transients in dendritic spines of these neurons in response to caffeine allowed us to demonstrate an independent and unique calcium store in spines. The response to caffeine was blocked by thapsigargin and ryanodine, and maintained in calcium‐free medium. The calcium stores were depleted faster in the spines than the dendrites. Furthermore, when calcium was released from stores under calcium‐free conditions, and diffused passively between the spine and the dendrite, the length of the spine neck determined the degree of spine independence. Finally, the caffeine‐sensitive ryanodine receptor‐linked calcium store was instrumental in regulating the response of neurons to glutamate. These results have important implications for understanding the roles of dendritic spines in neuronal integration and plasticity.