Open AccessDelivery of a healthy newborn using vitrified zygotes that developed from in vitro matured oocytes retrieved from a patient with polycystic ovarian syndrome
Author(s) -
Yoshida Hiroaki,
Aono Nobuya,
Araki Yasuhisa,
Naganuma Takako
Publication year - 2003
Publication title -
reproductive medicine and biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.005
H-Index - 22
eISSN - 1447-0578
pISSN - 1445-5781
DOI - 10.1046/j.1445-5781.2003.00025.x
Subject(s) - andrology , oocyte , human chorionic gonadotropin , zygote , polycystic ovary , embryo transfer , in vitro fertilisation , gynecology , equine chorionic gonadotropin , medicine , biology , embryo , embryogenesis , ovulation , hormone , microbiology and biotechnology , insulin , insulin resistance
The aim of this study is to evaluate vitrified zygotes, which were developed from in vitro matured oocytes that were retrieved from a patient with polycystic ovarian syndrome. Oocyte retrieval was performed on day 15 following withdrawal bleeding. The oocytes were incubated for 24 h in human tissue culture medium (TCM)‐199 maturation medium supplemented with 20% filtrated‐mixed patients follicle fluid, follicle stimulating hormone (FSH), and human chorionic gonadotropin (hCG). A total of four immature oocytes were collected. Two of the four oocytes (50.0%) developed to the metaphase‐II stage and, subsequently, two fertilized oocytes were vitrified at the pronuclear stage because a very thin endometrium was not conducive for transfer. One of the two vitrified zygotes was thawed and developed to a 4‐cell cleavage stage embryo within 24 h. Subsequent to the embryo transfer, a healthy newborn was delivered. A successful delivery was ensued by using vitrified zygotes from an anovulatory woman with polycystic ovarian syndrome. (Reprod Med Biol 2003; 2 : 87–90)