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Endoscopic 13 C‐urea breath test
Author(s) -
Urita Yoshihisa,
Miki Kazumasa
Publication year - 2000
Publication title -
digestive endoscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.5
H-Index - 56
eISSN - 1443-1661
pISSN - 0915-5635
DOI - 10.1046/j.1443-1661.2000.00006.x
Subject(s) - medicine , helicobacter pylori , gastroenterology , urea breath test , breath test , urea , urease , biopsy , stomach , rapid urease test , gastric mucosa , helicobacter infections , helicobacter pylori infection , biochemistry , chemistry
Background : Several modifications of the 13 C‐urea breath test (UBT) for the diagnosis of Helicobacter pylori infection have been published. A new modification of UBT is described with the aim of avoiding the influence of urease activities from the commensal organisms other than H. pylori upon 13 CO 2 excretion in the breath. Methods : The new modified UBT was performed in 295 consecutive patients undergoing esophagogastroscopy. After the collection of a baseline breath sample, an endoscopy was carried out. A baseline gastric gas sample was collected through a biopsy channel, and 20 mL of sterile water containing 100 mg of 13 C‐urea was sprayed onto the gastric mucosa. The scope was drawn after spraying the 13 C‐urea solution and collecting a gastric gas sample. Breath samples were collected 5, 10, 20 and 30 min after the spraying of 13 C‐urea. Results : The mean ± SD value of gastric gas samples for excess δ 13 CO 2 in H. pylori ‐positive patients was 1036.7 ± 1309.0‰, and 6.0 ± 23.3‰ in H. pylori ‐negative patients. The cut‐off point as determined by receiver operator characteristic (ROC) curves for the gastric gas samples was 10‰. The sensitivity and specificity for this new method were 95.4% and 92.5%, respectively. Conclusion : The method of endoscopically collecting 13 CO 2 gas in the stomach, which is not affected by the metabolism or by the absorption of 13 C, provides an accurate and sensitive means for the detection of H. pylori .