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Further investigation of the epitope recognized by the new monoclonal antibody 2C9
Author(s) -
KATO KEITARO,
YOSHIKAWA KAZUHIRO,
TAKI TOMOHIRO,
SHITARA KENYA,
NAKAMURA KAZUYASU,
HIROTA MAIKO,
HANAI NOBUO,
NAKAMURA KOGENTA,
KOKUBO HIROTO,
MITSUI KENJI,
YAMADA YOSHIAKI,
HONDA NOBUAKI,
UEDA RYUZO,
SAGA SHINSUKE,
FUKATSU HIDETOSHI
Publication year - 2003
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1046/j.1442-2042.2003.00655.x
Subject(s) - medicine , epitope , monoclonal antibody , virology , computational biology , antibody , immunology , biology
Objective: We established a new monoclonal antibody (2C9) that reacted with prostate tissue. The immunohistochemical reactivity of this antibody is similar to anti‐prostate‐specific membrane antigen (PSMA). Herein, we report the antigenic determinant of 2C9 antibody. Methods: The reactivity of the antibody was characterized by immunohistochemical staining and the antigen target was characterized by amino acid sequencing after immuno‐affinity purification from an LNCaP cell lysate and cloning of a cDNA using a mammalian expression cDNA cloning system. Results: The amino acid and nucleotide sequences for the antigen molecule recognized with 2C9 monoclonal antibody demonstrated identity with PSMA. Conclusion: The target molecule of the 2C9 monoclonal antibody is PSMA, pointing to future diagnostic and therapeutic applications.