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Immunological protection induced by bacillus Calmette–Guérin treatment in a murine bladder tumor model
Author(s) -
Iwasaki Akio,
Kawai Koji,
Hayashi Hitoshi,
Ikeda Noriko,
Toida Ichiro,
Ohtani Mikinobu,
Akaza Hideyuki
Publication year - 2002
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1046/j.1442-2042.2002.00449.x
Subject(s) - medicine , splenocyte , vaccination , immunotherapy , bladder tumor , bcg vaccine , antigen , inoculation , spleen , ratón , immunology , immune system , bladder cancer , cancer
Background : It has been previously reported that MBT‐2 tumor growth is completely inhibited when mice are inoculated with bacillus Calmette–Guérin (BCG). In this study it was examined whether or not vaccination with a mixture of BCG and MBT‐2 cells also induces immunological protection against murine bladder tumors.Methods : Seven hundred thousand MBT‐2 cells and 1 mg of BCG (Tokyo172 strain) per mouse were injected subcutaneously into female C3H/HeN mice. Four and eight weeks after vaccination with this mixture, animals were reinoculated with MBT‐2 cells alone or MBT‐2 cells cocultured with BCG.Results : Animals vaccinated with a mixture of BCG and MBT‐2 cells showed MBT‐2 tumor growth but completely rejected the MBT‐2 cells cocultured with BCG. MBT‐2 cells cocultured with BCG developed into tumors when they were inoculated into the control animals. Splenocytes prepared from vaccinated animals showed specific cytocidal activity against MBT‐2 cells precultured with BCG.Conclusions : The results suggest that a mixture of BCG and MBT‐2 cells induces antitumor immunological protection against BCG‐ or MBT‐2‐associated antigens presented on MBT‐2 cells precultured with BCG.