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GnRH analog, leuprorelin acetate, promotes regeneration of rat spermatogenesis after severe chemical damage
Author(s) -
Udagawa Koichi,
Ogawa Takehiko,
Watanabe Takeshi,
Yumura Yasushi,
Takeda Mitsumasa,
Hosaka Masahiko
Publication year - 2001
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1046/j.1442-2042.2001.00382.x
Subject(s) - leuprorelin , medicine , spermatogenesis , busulfan , chemotherapy , urology , lesion , endocrinology , hormone , andrology , surgery , cyclophosphamide , gonadotropin releasing hormone , luteinizing hormone
Background: Future fertility is a major concern for cancer patients who undergo intensive chemotherapy. There has been controversy about whether hormonal treatments may have protective effects against the severe spermatogenic damage caused by chemotherapy or irradiation. Recently, it has been proposed that gonadotrophin‐releasing hormone (GnRH) analogs administered after testicular damage stimulate the recovery of spermatogenesis. In this study, we have investigated the effects of GnRH agonist, leuprorelin, on the damage to spermatogenesis induced by busulfan. Methods: Fisher rats were treated with busulfan, 25 mg/kg, intraperitoneally. The effects of subcutaneous injections of leuprorelin before or after treatment were evaluated histologically 18 weeks later. Results: The percentage of ‘recovered’ seminiferous tubules was 27.7 ± 12.6% in control rats without leuprorelin and 26.9 ± 10.2% in rats with leuprorelin injected 4 weeks before busulfan. Rats in both groups showed poor recovery of spermatogenesis with an increase of intratesticular fluid. However, rats treated with leuprorelin three times (4 weeks apart) after busulfan showed an improvement of up to 56.5 ± 12.0% ( P < 0.05). A focal but massive necrotic lesion in the testis was observed only in this group of rats. Conclusions: The results demonstrated that leuprorelin administered after chemical testicular damage enhanced the recovery of spermatogenesis. At the same time, a possible significant side‐effect of leuprorelin was noted.