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Effect of prostatic neuropeptides on migration of prostate cancer cell lines
Author(s) -
Nagakawa Osamu,
Ogasawara Masaru,
Murata Jun,
Fuse Hideki,
Saiki Ikuo
Publication year - 2001
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1046/j.1442-2042.2001.00250.x
Subject(s) - lncap , vasoactive intestinal peptide , prostate cancer , neuropeptide , endocrinology , calcitonin , medicine , prostate , bombesin , gastrin releasing peptide , neuropeptide y receptor , cancer cell , gastrin , calcitonin gene related peptide , cancer research , cancer , secretion , receptor
Background:A previous study by the same authors demonstrated that among various neuropeptides in the prostate, calcitonin gene‐related peptide (CGRP) and gastrin‐releasing peptide (GRP) increased the invasive capacity of PC‐3 prostate cancer cells through enhancement of cell motility, while substance P (SP) inhibited the invasiveness through suppression of motile response.Methods:The effect of 10 kinds of neuropeptides were investigated, including CGRP, GRP, SP, neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP), calcitonin (CT), leucine‐enkephalin (L‐ENK), methionine‐enkephalin (M‐ENK), glucagon and parathyroid hormone‐related protein (PTH‐rP), on the invasion of DU‐145 prostate cancer cells through a reconstituted basement membrane (Matrigel) and the haptotactic migration of DU‐145, TSU‐pr1 and LNCaP prostate cancer cells using a Transwell cell culture chamber assay.Results:It was found that GRP, CGRP and PTH‐rP increased the invasive capacity of tumor cells. In contrast, SP, VIP, CT, L‐ENK, M‐ENK, NPY and glucagon had no significant effect. These three neuropeptides also increased the haptotactic migration of tumor cells to fibronectin. In addition VIP, CGRP and GRP increased the haptotactic migration of LNCaP prostate cancer cells and GRP and PTH‐rP increased the migration of TSU‐pr1 cells.Conclusion:The results indicated that some prostatic neuropeptides increased the invasive potential of prostate cancer cells partially through enhancement of cell motility.