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Characterization of a newly established cell line derived from human adrenocortical carcinoma
Author(s) -
Ueno Munehisa,
Nakashima Jun,
Akita Masumi,
Ban ShinIchi,
Nakanoma Takashi,
Iida Masahiro,
Deguchi Nobuhiro
Publication year - 2001
Publication title -
international journal of urology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.172
H-Index - 67
eISSN - 1442-2042
pISSN - 0919-8172
DOI - 10.1046/j.1442-2042.2001.00238.x
Subject(s) - adrenocortical carcinoma , doubling time , adrenal cortex , pathology , cell culture , carcinogenesis , medicine , carcinoma , adrenal gland , population , cancer research , microbiology and biotechnology , endocrinology , biology , cancer , genetics , environmental health
Background: ACT‐1, a new cell line of human adrenocortical carcinoma, has been established and successfully maintained in culture. This study examined the biological characteristics of the cells. Methods: The tumor cells were isolated from a surgical specimen of the tumor thrombus and cultured in monolayer. Results: Histologically, the primary tumor was composed of a solid proliferation of large polygonal cells. A part of the atrophic adrenal cortex remained at the periphery of the tumor. The cultured ACT‐1 cells were spindle‐shaped in morphology and grew exponentially with an approximate population doubling time of 24 h. A chromosomal analysis revealed a modal number of 61 with consistent structural abnormalities of add(3)(q11), add(9)(p11), and add(16)(q11). The expression of 3β‐hydroxysteroid dehydrogenase was observed in the ACT‐1 cells as well as in normal human adrenal glands. Conclusions: The ACT‐1 cell line provides a reproducible model system which gives good insight into the oncogenesis of adrenocortical carcinoma.