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Regulation of CD31 expression and interleukin‐4 production by human cord blood CD4 + T cells with interleukin‐4 and interleukin‐7
Author(s) -
Katamura, Kenji,
Fukui, Tetsuya,
Kiyomasu, Takahiro,
Ohmura, Kayo,
Iio Jun,
Ueno Hideki
Publication year - 2000
Publication title -
pediatrics international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.49
H-Index - 63
eISSN - 1442-200X
pISSN - 1328-8067
DOI - 10.1046/j.1442-200x.2000.01194.x
Subject(s) - interleukin 4 , interleukin 21 , immunology , interleukin 15 , medicine , t cell , cord blood , interleukin , t cell receptor , interleukin 2 , interleukin 3 , priming (agriculture) , microbiology and biotechnology , cytokine , immune system , biology , botany , germination
Background: T helper 2 cell type cytokines, such as interleukin (IL)‐4 and IL‐5, play pivotal roles in the development of allergic diseases. However, the mechanism by which naive CD4 + T cells acquire the ability to produce these cytokines remains unclear. Recently, it was reported that IL‐7 induces the ability to produce IL‐4 as well as interferon (IFN)‐γ and IL‐5 in naive CD4 + T cells without TCR stimulation. To further analyze the mechanism of acquiring IL‐4‐producing ability by naive CD4 + T cells, the effects of IL‐7 on human cord blood CD4 + T cells were compared with those of IL‐4, which induced the ability to produce IFN‐γ but not IL‐4.Results: Interleukin‐7 preserved the population of CD4 + CD31 – T cells in cord blood and induced their IL‐4‐producing ability without T cell receptor (TCR) stimulation, while IL‐4 induced CD31 on CD31 – T cells and could not induce their IL‐4‐producing ability. Both the CD31‐inducing effect and the inhibitory priming effect for IL‐4‐production by IL‐4 were also observed after cord blood CD4 + T cells had been primed with IL‐7 and acquired the IL‐4‐producing ability.Conclusions: Interleukin‐7 induced the IL‐4‐producing ability in naive CD4 + CD31 – T cells without TCR stimulation, suggesting that the signal transduction via CD31 may have an inhibitory effect on the acquisition of the IL‐4‐producing ability by cord blood CD4 + T cells in the absence of TCR stimulation.