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Evidence for neutrophil activation in occupational asthma
Author(s) -
Jung KiSuck,
Park HaeSim
Publication year - 1999
Publication title -
respirology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 85
eISSN - 1440-1843
pISSN - 1323-7799
DOI - 10.1046/j.1440-1843.1999.00196.x
Subject(s) - medicine , asthma , bronchoconstriction , eosinophil cationic protein , toluene diisocyanate , eosinophil , immunology , sputum , myeloperoxidase , inflammation , pathology , chemistry , tuberculosis , organic chemistry , polyurethane
Abstract In order to evaluate the role of neutrophils in the pathogenesis of occupational asthma (OA), 15 toluene diisocyanate (TDI)‐asthma and six grain dust‐asthma patients were recruited. Controls were the same number of subjects showing negative bronchoprovocation test (BPT) and six house dust mite‐sensitive asthma. Bronchoscopic biopsy specimens were stained with monoclonal antibodies to mast cell (AA1), eosinophil (EG2), pan T cell (CD3) and neutrophil (NE). Serum neutrophil chemotactic activity (NCA) was measured before and 10–420 min after BPT. Sputum interleukin‐8 (IL‐8) and myeloperoxidase (MPO) were also measured. There was a significant increase of NE + cells as well as AA1 + and EG2 + cells in grain dust‐ and TDI‐asthma compared with house dust‐sensitive asthma ( P < 0.05). Neutrophil + cells and AA1 + cells showed a significant correlation in TDI‐asthma ( r = 0.73, P = 0.02). Serum NCA was significantly increased at 10 min after BPT and decreased at 60 min in subjects with TDI‐asthma. In grain dust‐asthma, serum NCA increased at 30 min and decreased at 240 min after BPT ( P < 0.05). Sputum IL‐8 and MPO were significantly increased after BPT in both TDI‐ and grain dust‐asthma ( P < 0.05). These findings suggested that neutrophils in the lungs might contribute to bronchoconstriction induced by either TDI or grain dust. The possible involvement of IL‐8 in activation of neutrophils was also suggested.