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Mariculture of Laminaria japonica (Laminariales, Phaeophyceae) using protoplast regeneration
Author(s) -
Matsumura Wataru,
Yasui Hajime,
Yamamoto Hirotoshi
Publication year - 2000
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1046/j.1440-1835.2000.00204.x
Subject(s) - sporophyte , protoplast , biology , botany , laminaria , gametophyte , mariculture , algae , regeneration (biology) , japonica , epidermis (zoology) , microbiology and biotechnology , fishery , aquaculture , anatomy , fish <actinopterygii> , pollen
SUMMARY The conditions for culture of viable protoplasts from Laminaria japonica were investigated and the regenerative processes were observed in detail. As a result of culturing at four water temperatures (5, 10, 15, and 18°C), we found that low water temperature was better for survival, division and rhizoidal formation of protoplast‐derived cells. Only epidermis‐derived protoplasts developed into normal sporophytes through a direct developmental process. Protoplast‐derived cells divided after 5 days and 2–10 celled germlings formed the first rhizoids after 15 days. Only initial sporophytes with the first rhizoids grew to normal sporophytes with multilayered blades, stipes and holdfasts. When these young sporophytes were transplanted into the sea, they grew to normal fertile sporophytes.