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Isolation of disproportionating enzyme from halotolerant microalga Dunaliella parva (Volvocales, Chlorophyceae)
Author(s) -
Hatanaka Yoshiro,
Inaoka Kokoro,
Kobayashi Osamu,
Higashihara Masataka,
Hiyama Keiichiro
Publication year - 2000
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1046/j.1440-1835.2000.00178.x
Subject(s) - dunaliella salina , biology , dunaliella , halotolerance , chlorophyceae , enzyme , biochemistry , isozyme , chlamydomonas reinhardtii , enzyme assay , starch , chlamydomonas , algae , botany , chlorophyta , salinity , gene , ecology , mutant
SUMMARY Starch metabolism in Dunaliella parva Lerche is regulated by the osmolarity of the surrounding solute. Two isozymes showing amylolytic activity were obtained after purification by gel filtration chromatography. The isozymes show disproportionating activity (D‐enzyme) that is specific for malto‐oligosaccharides as substrate. Properties of the D‐enzyme in D. parva are similar to those in higher plants. The activity of the D‐enzyme is also found in various Dunaliella and Chlamydomonas , indicating that the D‐enzyme is also important in the starch metabolism in algae.