Premium
Screening of epiphytic dinoflagellates for radical scavenging and cytotoxic activities
Author(s) -
Nakagawa Hidenori,
Murata Michio,
Tachibana Kazuo,
Shiba Tadayoshi
Publication year - 1998
Publication title -
phycological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.438
H-Index - 44
eISSN - 1440-1835
pISSN - 1322-0829
DOI - 10.1046/j.1440-1835.1998.00116.x
Subject(s) - biology , antioxidant , cytotoxic t cell , biochemistry , lipid peroxidation , botany , in vitro
SUMMARY In the search for antioxidant substances/radical scavengers and cytotoxic substances, extracts from four species (10 strains) of epiphytic dinoflagellates in laboratory cultures were subjected to screening for production of bioactive metabolites. Assays for antioxi‐dants were performed using microsomal lipids prepared from rat livers, which were oxidized with Fe 3+ ‐nitrilotriacetic acid complex (Fe 3+ ‐NTA). Generated lipid peroxides were determined using the thiobarbituric acid (TBA) colorimetric method. Screening tests for cytotoxic activity were carried out using P388 leukemic cells of which the survival ratio was assessed using the tetrazolium salt (MTT) method. Extracts from two strains ( Gymnodinium sp. and Gambierdiscus toxicus ) and those from five strains (two of G. toxicus , two of Coolia monotis , and one of Prorocentrum sp.) were found to contain active constituents for antioxidant activity and for cytotoxic activity, respectively.