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Immunohistochemical analysis for histopathological subtypes in pediatric medulloblastomas
Author(s) -
Son EunIk,
Kim IlMan,
Kim DongWon,
Yim Man Bin,
Kang YuNa,
Lee SangSook,
Kwon KunYoung,
Suh SeongIl,
Kwon TaegKyu,
Lee JungJeung,
Kim DongSug,
Kim SangPyo
Publication year - 2003
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.2003.01444.x
Subject(s) - immunohistochemistry , synaptophysin , pathology , glial fibrillary acidic protein , anaplasia , neural cell adhesion molecule , medulloblastoma , biology , medicine , cell , cell adhesion , genetics
Medulloblastomas occurring in children represent a histological spectrum of varying anaplasia and nodularity. In order to determine whether immunohistochemical markers might be useful parameters in subclassifying these tumors, 17 pediatric medulloblastomas, including nine diffuse/non‐anaplastic, four diffuse/anaplastic, three nodular/non‐anaplastic and one nodular/anaplastic subtypes, were studied. In the present report, we investigate the expression of neural cell adhesion molecule (NCAM), nerve growth factor receptor (NGFR), neurofilament (NF), synaptophysin (SYN), glial fibrillary acidic protein (GFAP), S100, Bcl‐2, and Ki‐67 by using the immunohistochemistry against specific antibodies. This study showed that NGFR, NF, GFAP and S100 were not detected in anaplastic subtypes of medulloblastomas (0/5), while non‐anaplastic subtypes were mainly expressed within the nodules. All 17 tumors were reactive for NCAM, SYN and Bcl‐2. In addition, Ki‐67 labeling indices for anaplastic subtypes (39.0 ± 7.42%) were significantly higher than that of non‐anaplastic medulloblastomas (11.4 ± 8.04%; P < 0.0001). These results suggest that immunohistochemical markers are a useful adjunct in characterizing subtypes of pediatric medulloblastomas.