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Quantitative immunohistochemical evaluation of MIB‐1 labeling index in adult soft‐tissue sarcomas by computer‐assisted image analysis
Author(s) -
Hasegawa Tadashi,
Yamamoto Seiichiro,
Matsuno Yoshihiro
Publication year - 2002
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.2002.01378.x
Subject(s) - soft tissue , grading (engineering) , pathology , medicine , immunohistochemistry , kappa , sarcoma , immunostaining , gold standard (test) , tissue microarray , nuclear medicine , radiology , biology , mathematics , ecology , geometry
We have found that the MIB‐1 grade, based on tumor differentiation/histological type, necrosis and Ki‐67 (MIB‐1) score, is a valid and reproducible prognostic system for adult soft‐tissue sarcomas. However, there are limited data available on Ki‐67 labeling indices (LI) from adult soft‐tissue sarcomas for testing the validity of quantitative image analysis . In this study, the records of 146 adult patients with soft‐tissue sarcomas of the extremities and trunk were retrieved, and MIB‐1 immunostaining was carried out for the grading. The counted MIB‐1 LI values and the scores estimated from microscopic observation were defined as the gold standard. The correlation between MIB‐1 LI as assessed by computer‐assisted image analysis and by microscopic observation was determined. The image analysis ‐based MIB‐1 LI was highly correlated with the microscopic observation‐based MIB‐1 LI ( r = 0.87, 95% confidence interval (CI) = 0.82–0.92). In addition, agreement between the MIB‐1 scores was very high (kappa statistic = 0.83, 95% CI = 0.75–0.91), as was the percentage agreement (89%, 95% CI = 82.8–93.6%) between the results from image analysis and microscopic observation. We conclude that quantitative immunohistochemical evaluation of MIB‐1 LI by image analysis enables pathologists to improve interobserver agreement in the assessment of MIB‐1 score, and can help to objectively assign the correct histological grade to cases of adult soft‐tissue sarcoma, resulting in optimal clinical management.