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Differential distribution of basement membrane type IV collagen α1(IV), α2(IV), α5(IV) and α6(IV) chains in colorectal epithelial tumors
Author(s) -
Hiki Yutaka,
Iyama Kenichi,
Tsuruta Junji,
Egami Hiroshi,
Kamio Takihiro,
Suko Shuji,
Naito Ichiro,
Sado Yoshikazu,
Ninomiya Yoshifumi,
Ogawa Michio
Publication year - 2002
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.2002.01341.x
Subject(s) - pathology , immunostaining , immunohistochemistry , basement membrane , atypia , epithelium , colorectal cancer , chemistry , biology , medicine , cancer
In this study, we examined the relationship between the histopathological grade and immunohistochemical localization of six genetically distinct type IV collagen α chains, the major component of basement membrane (BM), in normal and neoplastic colorectal tissues. In the normal colorectal mucosa, α1/α2(IV) and α5/α6(IV) chains were stained in all epithelial BM. However, α3/α4(IV) chains were restrictively immunostained in the BM of the apical surface epithelium. Similar immunostaining profiles for α1/α2(IV) and α5/α6(IV) chains were observed in tubular adenomas with mild/moderate atypia. However, in intramucosal carcinomas, both α1/α2(IV) chains were linearly stained in the BM of cancer cell nests, while the assembly of α5/α6(IV) chains into the BM was inhibited in a discontinuous or negatively stained pattern. The normal colorectal mucosa forms a second network of BM composed of α5/α6(IV), partly α3/α4(IV) chains, in addition to the classic network of α1/α2(IV) chains. The differential immunohistochemical localization of the type IV collagen α5/α6 chains could be one diagnostic marker for the invasiveness of colorectal cancer.