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Establishment and characterization of three new rat renal cell carcinoma cell lines from N ‐ethyl‐ N ‐hydroxyethylnitrosamine‐induced basophilic cell tumors
Author(s) -
Tokuzen Reiko,
Iwahori Yoshio,
Asamoto Makoto,
Iigo Masaaki,
Hasegawa Fumio,
Satoh Takatomo,
Ishidate Motoi,
Tsuda Hiroyuki
Publication year - 2001
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.2001.01167.x
Subject(s) - basophilic , cytoplasm , cell culture , biology , microbiology and biotechnology , cell , carbonic anhydrase , intracellular , pathology , biochemistry , chemistry , enzyme , genetics , medicine
Three new rat cell lines (designated as BP13, BP30 and BP36B), derived from rat basophilic‐type renal cell carcinomas induced with N ‐ethyl‐ N ‐hydroxyethylnitrosamine, were established and characterized. Passaged up to 100 times in vitro for 3 years, each cell line forms epithelial monolayers with cell cycles for BP13, BP30 and BP36B of 29, 21 and 17 h, respectively. Positive glucose‐6‐phosphate dehydrogenase (G6PD) and γ‐glutamyltransferase (γ‐GT) activity in their cytoplasm, but negative succinate dehydrogenase (SD) and slightly positive carbonic anhydrase type II (CA) localization indicates an origin from proximal tubules. Ultrastructural examination showed the presence of variable numbers of mitochondria and many microvilli and intracellular junctions on the plasma membrane. BP13 and BP30 were found to be tetraploid and BP36B diploid. BP13 has one marker chromosome 15p+, and BP36B an isochromosome of 1q. Anchorage‐independent growth and tumorigenicity in immunosuppressed nude mice of BP13 and BP36B, but not BP30, proved their neoplastic nature. These three cell lines should provide useful tools for studying the biological characteristics of renal cell tumors.