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A human B‐lineage dendritic cell line, HBM‐Noda and its potential role in human T‐cell leukemia/lymphoma virus type I infection
Author(s) -
Nagasaki Makoto,
Morikawa Shigeru,
Torii Ikuko,
Zhang Jie,
Morikawa Keiko
Publication year - 2000
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.2000.01040.x
Subject(s) - biology , virology , antigen , leukemia , virus , lymphoma , flow cytometry , cell culture , dendritic cell , tropism , immunology , genetics
An Epstein–Barr virus‐transformed B‐cell line, HBM‐Noda (Noda), that has a dendritic morphology as well as several characteristic features of dendritic cells (DC) has been established. We therefore refer to Noda as B‐lineage DC. Although human T‐cell leukemia/lymphoma virus type I (HTLV‐I) exhibit substantial cellular tropism, the roles of DC in HTLV‐I infection remain unknown. To further clarify the characteristics of Noda cells, we performed infection experiments using a concentrated HTLV‐I fraction from the adult T‐cell leukemia cell line, HPB‐ATL‐2. Noda, as well as other cell lines examined, were sensitive to HTLV‐I infection as detected by proviral DNA using polymerase chain reaction, but most infected Noda cells underwent necrosis within 7 days. The most striking feature of Noda cells was the abundant expression of viral antigen (p19) on the cell surface following infection (approximately day 4), probably due to strong viral adsorption. In cocultivation experiments using Noda cells at day 1 of post‐infection and peripheral blood activated T cells, we detected a few (1.3%) viral antigen expressing T cells after 5 days of coculture by flow cytometry. These results suggest that B‐lineage DC such as Noda cells play a role in the establishment of HTLV‐I infection at an early phase.