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Semiquantitative immunoblot analysis of nm23‐H1 and ‐H2 isoforms in adenocarcinomas of the lung: Prognostic significance
Author(s) -
Sato Yuichi,
Tsuchiya Benio,
Urao Takeshi,
Baba Hideo,
Shiku Hiroshi,
Kodama Tetsuro,
Kameya Toru
Publication year - 2000
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.2000.01030.x
Subject(s) - adenocarcinoma , gene isoform , pathology , immunohistochemistry , stage (stratigraphy) , lung , pulmonary adenocarcinoma , monoclonal antibody , metastasis , frozen section procedure , biology , antibody , medicine , cancer , immunology , gene , paleontology , biochemistry
Total amounts of nm23 protein and relative levels of H1 and H2 isoforms were studied in 27 fresh‐frozen samples of pulmonary adenocarcinoma and adjacent non‐neoplastic tissues that were obtained at surgery. Semiquantitative immunoblotting with a monoclonal antibody (Pan‐242) against nm23 protein demonstrated both isoforms, recognized as 20.5 kDa for H1 and 18.5 kDa for H2, to be present in all cases. Both H1 and H2 levels in neoplastic tissues were higher than in the corresponding non‐neoplastic samples. Expression of H2 was usually greater than of H1. The H2/H1 ratio varied from 1.9 to 14.1 (mean value 5.2) in non‐neoplastic tissues and 1.0–5.9 (mean value 2.5) in neoplastic tissues, although this ratio did not correlate with any prognostic factor like tumor size, nodal status or distant metastasis (TNM tumor stage). H1 and H2 levels were significantly lower (mean values 4.3 and 2.4) in well‐differentiated than in moderately and poorly differentiated adenocarcinomas (8.3 and 3.0) ( P < 0.03 and P < 0.05, respectively). These data indicate that H1 and H2 isoform levels correlate with histological differentiation, but not the metastatic potential or stage of pulmonary adenocarcinoma.