Premium
Expression of scavenger receptor class A and CD14 in lipopolysaccharide‐induced lung injury
Author(s) -
Yamamoto Takashi,
Ebe Yusuke,
Hasegawa Go,
Kataoka Masashi,
Yamamoto Shunsuke,
Naito Makoto
Publication year - 1999
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.1999.00978.x
Subject(s) - scavenger receptor , cd14 , lipopolysaccharide , receptor , macrophage , alveolar macrophage , apoptosis , lung , cytokine , inflammation , immunology , medicine , biology , endocrinology , chemistry , in vitro , biochemistry , lipoprotein , cholesterol
CD14 and macrophage scavenger receptor class A type I and II (MSR‐A) are receptors for lipopolysaccharide (LPS). In this study, the expressions of both receptors in the lung after administration of LPS in aerosol to mice with a nebulizer were observed. Bronchiolar epithelial cells and alveolar macrophages immediately incorporated LPS and expressed CD14. CD14‐positive neutrophils then appeared in the alveolar space followed by the appearance of MSR‐A‐expressing cells in the vascular lumen, pulmonary interstitium, and alveolar space. Numbers of apoptotic cells increased after 1 day, and MSR‐A‐expressing macrophages actively incorporated apoptotic bodies. Daily administration of macrophage colony stimulating factor (M‐CSF) to the mice resulted in increased levels of MSR‐A expression and reduced levels of CD14 as well as several cytokine expressions, leading to shortening of the inflammatory process. The numbers of apoptotic cells were reduced in M‐CSF injected mice. These findings imply that CD14 acts as an immediate expressing receptor for LPS and MSR‐A exerts a protective function by scavenging LPS and apoptotic cells in LPS‐induced lung injury.