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Overexpression and genetic abnormality of p53 in parathyroid adenomas
Author(s) -
Kishikawa Seigo,
Shan Liang,
Ogihara Kikumi,
Utsunomiya Hirotoshi,
Nakamura Misa,
Nakamura Yasushi,
Naito Akihiro,
Kakudo Kennichi
Publication year - 1999
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.1999.00961.x
Subject(s) - pleomorphism (cytology) , pathology , immunohistochemistry , malignancy , abnormality , single strand conformation polymorphism , parathyroid neoplasm , biology , adenoma , exon , parathyroid adenoma , medicine , gene , genetics , psychiatry
To study the significance of p53 abnormality in parathyroid tumors, 32 parathyroid adenomas and 22 hyperplastic glands from 14 cases of secondary hyperparathyroidism were analysed using immunohistochemistry, polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP), single‐strand conformation polymorphism (SSCP) and DNA sequencing. Immunohistochemical study revealed p53 overexpression in four parathyroid adenomas, of which two showed diffuse and one showed focal nuclear pleomorphism. Genetic analysis disclosed allelic loss in one, and a point mutation (R290H) and a polymorphism (L257 L) in another of the two other adenomas with diffuse nuclear pleomorphism. No abnormalities were discovered in the other two adenomas, although one had a R72P polymorphism in exon 4. There was no evidence of malignancy of the four tumors in either clinical or pathological terms. None of the 22 hyperplastic glands showed p53 overexpression. These results demonstrate that p53 abnormality can occur in benign parathyroid adenomas and is more prevalent in those with nuclear pleomorphism than in those without.