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Phaseolus vulgaris leukoagglutinating lectin‐binding reactivity in human diffuse large B‐cell lymphoma and its relevance to the patient’s clinical outcome: Lectin histochemistry and lectin blot analysis
Author(s) -
Suzuki Osamu,
Nozawa Yoshihiro,
Kawaguchi Takanori,
Abe Masafumi
Publication year - 1999
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.1999.00960.x
Subject(s) - lectin , western blot , immunohistochemistry , glycoprotein , lymphoma , biology , blot , biochemistry , microbiology and biotechnology , chemistry , immunology , gene
Many reports have suggested a strong correlation between certain lectin‐binding patterns and biological behavior in various tumors. To clarify a relationship between lectin‐binding reactivity and survival of patients with diffuse large B‐cell lymphoma (B‐DLCL), 57 cases with B‐DLCL were analyzed by lectin histochemistry and lectin blot method with or without treatment of neuraminidase or acidic hydrolytic conditions. B‐DLCL cases were grouped into three types based on the data on lectin‐binding reactivity under neuraminidase‐treated or untreated conditions: (i) Group A (non‐reactive type); (ii) Group B (sialylated type); and (iii) Group C (non‐sialylated type). Among various lectins, Phaseolus vulgaris ‐ L (L‐PHA) binding reactivity showed that the survival of patients with Group A + B or Group B was significantly shorter than that of patients with Group C. Lectin blot analysis revealed failure of L‐PHA‐binding to 32 kd and 29 kd glycoproteins, which may be attributable to the masking of L‐PHA‐binding sites by sialylation or the lack of L‐PHA‐binding sites, leading to the short survival of patients with B‐DLCL. L‐PHA‐binding reactivity may be a useful marker for the evaluation of survival of patients with B‐DLCL.