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Localization of prostate‐specific antigen‐like immunoreactivity in human salivary gland and salivary gland tumors
Author(s) -
Tazawa Kenichi,
Kurihara Yuka,
Kamoshida Shingo,
Tsukada Kazuhiro,
Tsutsumi Yutaka
Publication year - 1999
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1046/j.1440-1827.1999.00900.x
Subject(s) - salivary gland , immunoperoxidase , ductal cells , pathology , submandibular gland , antigen , kallikrein , biology , prostate specific antigen , cytoplasm , prostate , immunohistochemistry , endocrinology , medicine , antibody , monoclonal antibody , cancer , immunology , enzyme , microbiology and biotechnology , biochemistry
Immunoreactivity of prostate‐specific antigen (PSA), a kallikrein‐like enzyme present in the seminal plasma, was demonstrated by indirect immunoperoxidase staining using a PSA antiserum in the apical cytoplasm along the luminal border of small‐sized duct epithelial cells of the major salivary (parotid and submandibular) gland of both sexes (56/56, 100%). No PSA‐like immunoreactivity was seen in large‐sized duct epithelial cells and acinar cells. Minor salivary gland ducts were negative. When inflammatory and atrophic changes were observed, ductal expression of PSA‐like immunoreactivity was decreased (12/37, 32%) and the site of intracellular localization often became diffusely cytoplasmic. The immunoreactivity was absorbed by human seminal plasma. Immunoreactivities of prostatic acid phosphatase and sex hormone receptors were undetectable in the salivary gland. Twenty‐nine (34%) of 86 salivary gland tumors with ductal differentiation were immunoreactive for PSA mainly in the cytoplasm. A PSA monoclonal antibody ER‐PR8 detected immunoreactivity in the prostate but not in the salivary glands or their tumors. Prostate‐specific antigen‐like immunoreactivity in small‐sized (intercalated) duct epithelial cells of the major salivary gland and their tumors may be due to cross‐reactivity of the antiserum with kallikrein‐like substances.