Premium
A newly developed assay for melatonin using cells expressing human mel‐1a receptor
Author(s) -
Ozeki Yuji,
Yamada Naoto,
Aoki Harusuke,
Yokoyama Tetsuo,
Shirono Hiroyuki,
Koga Jyunichi,
Kato Nobumasa
Publication year - 1999
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1046/j.1440-1819.1999.00482.x
Subject(s) - melatonin , chinese hamster ovary cell , radioimmunoassay , melatonin receptor , receptor , bioassay , hamster , radioligand assay , chemistry , biology , endocrinology , medicine , chromatography , biochemistry , genetics
We have developed a radioreceptor binding assay (RRA) method for melatonin using membranes from Chinese hamster ovary cells that can stably express human mel‐1a receptors. We measured melatonin levels in plasma samples collected every 4 h for 24 h using the RRA and radioimmunoassay (RIA) methods, simultaneously. There was a statistically significant correlation between the melatonin levels measured by the two methods, this newly developed method providing a sensitive bioassay. As it is possible to circumvent the cross‐reactivity usually occurring in the RIA method, this method may be an important tool for detecting bioactive substances relative to the mel‐1a receptor.