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Altered adhesion efficiency and fibronectin content in fibroblasts from schizophrenic patients
Author(s) -
Miyamae Yasuyuki,
Nakamura Yu,
Kashiwagi Yujiro,
Tanaka Toshihisa,
Kudo Takashi,
Takeda Masatoshi
Publication year - 1998
Publication title -
psychiatry and clinical neurosciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.609
H-Index - 74
eISSN - 1440-1819
pISSN - 1323-1316
DOI - 10.1046/j.1440-1819.1998.00386.x
Subject(s) - fibronectin , vimentin , vinculin , fibroblast , focal adhesion , chemistry , laminin , plectin , immunohistochemistry , microbiology and biotechnology , integrin , cell adhesion , blot , pathology , andrology , cell , medicine , biology , cytoskeleton , biochemistry , in vitro , intermediate filament , gene
Cultured fibroblasts from the cutaneous tissue of 16 schizophrenic patients were compared with 16 control cultured fibroblasts from the healthy subjects. The fibroblasts from the schizophrenic patients showed a decreased adhesion efficiency within 30 min after plating compared to that of the control subjects. However, after 90 min, there was no significant difference between the groups, where more than 90% of the cells from both groups had adhesed to the plate. By immunohistochemistry and western blotting using the antibodies against integrin (VLA5), talin, vinculin, fodrin, vimentin, ankyrin, plectin, fibronectin, and focal adhesion kinase (FAK), there was no significant difference in localization and amount between the groups. The amount of fibronectin released into the medium in which the fibroblast had already kept confluency showed no significant difference between the groups. However, the fibronectin content in cell lysate within 48 h after plating was significantly lower in the schizophrenic group.