Premium
Renal interstitial tenascin immunostaining and immune cell infiltration in IgA nephropathy
Author(s) -
Wa¸growskaDanilewicz Maĺgorzata,
Danilewicz Marian
Publication year - 2001
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1046/j.1440-1797.2001.00050.x
Subject(s) - medicine , cd68 , pathology , immunostaining , tenascin , immunoperoxidase , nephropathy , mesangium , renal biopsy , glomerulonephritis , infiltration (hvac) , biopsy , kidney , tenascin c , immunohistochemistry , monoclonal antibody , immunology , antibody , fibronectin , cell , biology , endocrinology , genetics , thermodynamics , physics , diabetes mellitus
SUMMARY: Interstitial expression of tenascin and interstitial leucocyte infiltration were examined by an indirect immunoperoxidase method using monoclonal antibodies against tenascin, CD45 (all leucocytes), CD45RO (T cells) and CD68 (monocytes/macrophages) on renal biopsy specimens from 25 patients with mesangial proliferative IgA‐positive glomerulonephritis (IgAN). Ten biopsy kidney specimens, which were removed because of renal trauma, were used as the control group. In patients with IgAN, the mean interstitial expression of tenascin was significantly higher than in the control group. Strong tenascin staining was detected in areas with interstitial damage. In patients with IgAN there were positive correlations between the interstitial expression of tenascin and the relative interstitial cortical volume, as well as serum creatinine. In the IgAN patents, a significant increase in the total number of interstitial CD45‐immunopositive cells, CD45RO‐positive and CD68‐positive cells was seen compared with the control group. In patients with IgAN, immunostaining of tenascin did not correlate with the number of T‐cells, monocytes/macrophages or all leucocytes in the renal interstitium. These results suggest that in patients with IgAN the interstitial accumulation of tenascin did not depend on the type or the density of interstitial inflammatory infiltrates.