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Polymorphonuclear leucocyte rheology and cytosolic Ca 2 + content after activation in chronic renal failure
Author(s) -
Caimi G,
Canino B,
Vaccaro F,
Montana M,
Carollo C,
Oddo G,
Presti R Lo
Publication year - 2001
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1046/j.1440-1797.2001.00049.x
Subject(s) - cytosol , medicine , endocrinology , chronic renal failure , phorbol , neutrophile , immunology , biochemistry , chemistry , protein kinase c , inflammation , enzyme
SUMMARY: We evaluated polymorphonuclear leucocyte (PMN) flow properties in patients with clinically stable chronic renal failure (CRF) and in control subjects at baseline and after activation with 4‐phorbol 12‐myristate 13‐acetate (PMA) and N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP). Initial relative flow rate (IRFR) and clogging particles (CPs) were obtained using the St. George's Filtrometer, and PMN membrane fluidity was assessed by marking PMNs with 1‐(4‐(trimethylamino)phenyl)‐6‐phenyl‐1,3,5‐hexatriene (TMA‐DPH). PMN cytosolic Ca 2+ concentration was determined by marking PMNs with Fura 2‐AM. At baseline, CRF patients showed a significant increase only in PMN cytosolic Ca 2+ content. After activation with PMA and fMLP, a decrease in IRFR and an increase in CP were observed in both control subjects and CRF patients, although the variation in IRFR present in the group of CRF patients was greater than in the control group. After activation with PMA and fMLP, we found a decrease in PMN membrane fluidity only in CRF patients, but no variation in PMN cytosolic Ca 2+ concentration in either group was observed. These results provide evidence for PMN dysfunction in chronic renal failure.