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Variable toxicity of dyes used in micropuncture studies
Author(s) -
NikolicPaterson David J,
Hurst Lynette A,
Comper Wayne D
Publication year - 2001
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1046/j.1440-1797.2001.00047.x
Subject(s) - toxicity , medicine , kidney tubules , in vitro , pharmacology , kidney , biochemistry , chemistry
SUMMARY: This study evaluated the potential toxicity of Fast green and Lissamine green, the two dyes commonly used for visualizing tubular fluid flow in micropuncture studies. In both the in vitro perfused kidney and cultured tubular epithelial cells, Fast green exhibited a profound, dose‐dependent toxicity. In contrast, Lissamine green was non‐toxic. In conclusion, we recommend that Fast green not be used in micropuncture studies.