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The prevention of glomerulosclerosis in rats using traditional Chinese medicine, Sairei‐to
Author(s) -
Li Ping,
Kawachi Hiroshi,
Suzuki Yasuhito,
Saito Akihiko,
Orikasa Michiaki,
Shi Zhen Sheng,
Shimizu Fujio
Publication year - 2000
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1046/j.1440-1797.2000.00503.x
Subject(s) - medicine , glomerulosclerosis , urology , proteinuria , kidney
SUMMARY: The effects of Sairei‐to on chronic irreversible lesions were examined by the administration of drugs after the initiation of irreversible renal lesions by uninephrectomy and anti‐thy‐1 antibody injection. Twenty‐one female Wistar rats were divided into three groups. Group 1 was treated with phosphate‐buffered saline (PBS) as a control and groups 2 and 3 were treated with Sairei‐to 400 mg/kg bodyweight per day on a daily basis beginning on day 1 (group 2) and day 7 (group 3) to day 42 after the intravenous administration of 500 μg anti‐thy‐1 monoclonal antibody (MoAb) into uninephrectomized rats, respectively. Statistically significant effects of Sairei‐to on proteinuria were observed on days 3, 7, 14, and 21 in group 2 and on day 21 in group 3 as compared with the PBS controls. On day 42, light microscopy showed that Sairei‐to ameliorated morphological lesions (Matrix score: 133.7 ± 50.35 for group 1 vs 40.0 ± 24.0 for group 2, P < 0.05). The weight of the kidneys in groups given Sairei‐to was also lower than that in the PBS control. In addition, immunofluorescent findings showed that Sairei‐to suppressed the expression of transforming growth factor‐beta (TGF‐β), alpha‐smooth muscle actin (α‐SMA) and collagen type I in glomeruli and decreased the number of ED1‐ and OX8‐positive cells in tubulo‐interstitium as well as in glomeruli. In conclusion, Sairei‐to blocked the progression of renal lesions in this model even when it was administered after the initiation of the disease. In addition, earlier treatment more effectively prevented the progression of the renal lesions.