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Effect of Sairei‐to on irreversible glomerular sclerotic lesions in rats
Author(s) -
LI Ping,
KAWACHI Hiroshi,
ORIKASA Michiaki,
Sheng SHI Zhen,
SHIMIZU Fujio
Publication year - 1998
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1046/j.1440-1797.1998.d01-3.x
Subject(s) - medicine , endocrinology , saline , urology , andrology
The effects of Sairei‐to and its active components on a model of irreversible mesangial proliferative glomerulonephritis induced by injecting monoclonal antibody (MoAb) 1‐22‐3 into uninephrectomized rats were examined. The significant suppressive effects of Sairei‐to and its active components on proteinuria were demonstrated on days 7, 14, 21 after MoAb 1‐22‐3 injection compared with phosphate‐buffered saline (PBS)‐treated controls. On day 21, light microscopy revealed that the drugs reduced mesangial cell proliferation, mesangial matrix expansion (matrix score: 84.5±41.6 for Sairei‐to, 76.1±31.9 for Syo‐saiko‐to, 66.7±46.3 for its three components vs 162.4±26.1 for PBS, P <0.005) and crescent formation (mean percentage: 2.25% for Sairei‐to, 1.71% for Syo‐saiko‐to, 1.43% for its three components vs 18.86% for PBS, P <0.005). The kidney weights of the groups given the drugs were significantly lower than the PBS group value (1.03±0.08 g with Sairei‐to, 1.11±0.12 g with Syo‐saiko‐to, 1.06±0.12 g with its three components vs 1.39±0.20 g with PBS, P <0.01 or P <0.05). Immunofluorescence analysis revealed that the drugs suppressed the expression of transforming growth factor‐β (TGF‐β), α‐smooth muscle actin (α‐SMA) and collagen type I in the glomeruli, and reduced the numbers of ED1‐positive cells in the glomeruli and OX8‐positive cells in the glomeruli and in the tubular interstitium. The blood biochemistry results revealed significant differences between the total cholesterol levels (70.0±5.9 mg/dL with Sairei‐to, 66.0±6.4 mg/dL with Syo‐saiko‐to, 76.6±8.4 mg/dL with its three components vs 104.3±26.6 mg/dL with PBS, P <0.05). We conclude that Sairei‐to and its active components have suppressive effects on proteinuria and mesangial matrix expansion in rats with irreversible renal sclerosis. Transforming growth factor‐β, collagen type I and α‐SMA expression and infiltration by ED1‐ and OX8‐positive cells were also suppressed by these drug preparations.