Premium
The effects of a high salt diet on gene expression of Na + /H + exchanger and growth factors in 5/6‐nephrectomized rats
Author(s) -
HIGASHI Harumichi,
OKUDA Seiya,
TAMAKI Kiyoshi,
ANDO Takashi,
FUJISHIMA Masatoshi
Publication year - 1998
Publication title -
nephrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.752
H-Index - 61
eISSN - 1440-1797
pISSN - 1320-5358
DOI - 10.1046/j.1440-1797.1998.d01-29.x
Subject(s) - amiloride , medicine , endocrinology , furosemide , sodium–hydrogen antiporter , glomerulosclerosis , sodium , muscle hypertrophy , kidney , chemistry , proteinuria , organic chemistry
SUMMARY: The effects of a high salt diet on renal destruction and the therapeutic effects of amiloride (Na + /H + exchanger inhibitor) and furosemide (Na + K + /2Cl − exchanger inhibitor) were examined in 5/6‐nephrectomized rats fed a high salt diet. A simultaneous analysis of the effects of a high salt intake on the renal expression of Na + /H + exchanger‐1 (NHE‐1), transforming growth factor‐β1 (TGF‐β1) or platelet‐derived growth factor‐B (PDGF‐B) mRNA was performed in this model. the 5/6‐nephrectomized Sprague‐Dawley rats were given a diet containing 8 or 1% sodium chloride for 5 weeks. This high salt diet accelerated the elevation of blood pressure and aggravated both glomerulosclerosis and interstitial fibrosis in 5/6‐nephrectomized rats. the daily administration of amiloride was found to be protective against the elevation of blood pressure, glomerular hypertrophy and the aggravation of renal histology which were induced by a high salt diet. the expression of TGF‐β1 and PDGF‐B mRNA was up‐regulated by a high salt diet, but the expression of NHE‐1 mRNA was not. the overexpression of TGF‐β1 and PDGF‐B mRNA was reduced by the daily administration of amiloride but not by furosemide. In conclusion, the destructive effects of a high salt diet on the kidneys may be mediated through hypertension, glomerular hypertrophy and the overexpression of the growth factors. Amiloride may thus be more protective for high salt induced renal aggravation than furosemide, although the expression of NHE‐1 mRNA did not show any substantial increase due to a high salt diet.