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The reaction of glial progenitor cells in remyelination following ethidium bromide‐induced demyelination in the mouse spinal cord
Author(s) -
Fushimi Shigeko,
Shirabe Teruo
Publication year - 2002
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1046/j.1440-1789.2002.00459.x
Subject(s) - remyelination , ethidium bromide , spinal cord , spinal cord injury , progenitor cell , bromide , progenitor , chemistry , medicine , neuroscience , biology , microbiology and biotechnology , stem cell , central nervous system , myelin , biochemistry , dna , organic chemistry
The present study investigated how glial progenitor cells participated in the process of remyelination following ethidium bromide (EBr)‐induced demyelination in the adult mouse spinal cord. In situ hybridization techniques for detecting mRNA for platelet‐derived growth factor α receptor (PDGFαR) and proteolipid protein (PLP) were employed to identify glial progenitor cells and mature oligodendrocytes, respectively. During the demyelination stage and early stage of remyelination, large cells strongly expressing PDGFαR mRNA were observed in the border of the demyelinating lesion, and with immunohistochemistry they exhibited positive labeling of the astrocytic marker glial fibrillary acidic protein (GFAP). Other glial progenitor cells expressing PDGFαR mRNA proliferated around the lesion during the demyelination stage. During the remyelination stage, some PDGFαR mRNA‐positive cells partly expressed mRNA for PLP in the periphery of the demyelinating lesion. These results suggest that PDGFαR mRNA‐positive glial progenitor cells may give rise to both astrocytes and oligodendrocytes, which participate in remyelination following demyelination.