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Ischemic rat brain extracts induce human marrow stromal cell growth factor production
Author(s) -
Chen Xiaoguang,
Li Yi,
Wang Lei,
Katakowski Mark,
Zhang Lijie,
Chen Jieli,
Xu Yongxian,
Gautam Subhash C.,
Chopp Michael
Publication year - 2002
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1046/j.1440-1789.2002.00450.x
Subject(s) - hepatocyte growth factor , stromal cell , neurotrophic factors , nerve growth factor , growth factor , vascular endothelial growth factor , neurotrophin , medicine , brain derived neurotrophic factor , ischemia , bone marrow , vascular endothelial growth factor a , endocrinology , pharmacology , vegf receptors , receptor
Intravenous administration of human bone marrow stromal cells (hMSCs) after middle cerebral artery occlusion (MCAo) in rats provides functional benefit. We tested the hypothesis that these functional benefits are derived in part from hMSC production of growth and trophic factors. Quantitative sandwich enzyme‐linked immunosorbent assay (ELISA) of hMSCs cultured with normal and MCAo brain extracts were performed. hMSCs cultured in supernatant derived from ischemic brain extracts increased production of brain‐derived neurotrophic factor (BDNF), nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF). These neurotrophins and angiogenic growth factors increased in a post‐ischemia time‐dependent manner. The hMSC capacity to increase expression of growth and trophic factors may be the key to the benefit provided by transplanted hMSCs in the ischemic brain.