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Age‐related changes of cornu ammonis 1 pyramidal neurons in gerbil transient ischemia
Author(s) -
Tamagaki Chiharu,
Murata Akira,
Asai Satoru,
Takase Katsunori,
Gonno Kazutoshi,
Sakata Tetsuji,
Kinoshita Toshihiko
Publication year - 2000
Publication title -
neuropathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 61
eISSN - 1440-1789
pISSN - 0919-6544
DOI - 10.1046/j.1440-1789.2000.00344.x
Subject(s) - gerbil , tunel assay , ischemia , apoptosis , hippocampal formation , programmed cell death , biology , lipofuscin , h&e stain , pathology , cathepsin b , anatomy , endocrinology , medicine , immunohistochemistry , enzyme , biochemistry
This study reports that postischemic apoptotic cell death of the hippocampal cornu ammonis (CA) 1 neurons is delayed in aged gerbils. Age‐related changes in the process of CA1 neuronal death following transient ischemia was studied. Two groups of Mongolian gerbils were used in this study, which compared adult (4‐month‐old) and aged (24‐month‐old) animals by hematoxylin–eosin stain, in situ nick‐end labeling (TUNEL method) and electron microscopy. In the process of neuronal death, neuronal loss of the aged group was histologically less severe than that of the adult group. TUNEL‐positive cells were found on days 3–5 after ischemia in the adult group, while they were still found on day 7 in the aged group. The apoptotic process of the aged group was delayed compared to the adult group. Furthermore, lipofuscin was ultrastructurally observed inside the apoptotic body 5 days after ischemia in CA1 pyramidal neurons of the aged group. It is likely that colocalization of lysosomal enzyme cathepsin D with lipofuscin might be associated with the age‐related alteration of lysosomal system in the neurons. Altogether these data suggest that age‐related lysosomal changes might affect the apoptotic cascade process in postischernic CA1 neurons.