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Prevalence of Helicobacter pylori resistance to clarithromycin and metronidazole determined by 23S ribosomal RNA and rdxA gene analyses in Hiroshima, Japan
Author(s) -
HIYAMA TORU,
TANAKA SHINJI,
MASUDA HIROSHI,
SHIMA HIDEYUKI,
KOSE KAZUHIRO,
TUNCEL HANDAN,
ITO MASANORI,
KITADAI YASUHIKO,
SUMII MASAHARU,
UEMURA NAOMI,
YOSHIHARA MASAHARU,
SHIMAMOTO FUMIO,
HARUMA KEN,
CHAYAMA KAZUAKI
Publication year - 2003
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2003.03140.x
Subject(s) - 23s ribosomal rna , clarithromycin , metronidazole , helicobacter pylori , microbiology and biotechnology , ribosomal rna , medicine , antibiotics , antibiotic resistance , gene , biology , gastroenterology , rna , genetics , ribosome
Background and Aims: Resistance to antibiotics in Helicobacter pylori is increasing and becoming a serious problem in eradication treatment of H. pylori . The prevalence of H. pylori infections that are resistant to clarithromycin, metronidazole, or both were determined in H. pylori isolates in Hiroshima, Japan. Methods: Sixty Japanese patients with H. pylori infection were collected between 1999 and 2000. To detect the resistance to clarithromycin and metronidazole, mutations of the 23S ribosomal RNA ( rRNA ) and rdxA genes that are responsible for resistance in H. pylori, were examined by direct sequencing analysis. Results: Resistance to clarithromycin and metronidazole was detected in 12 (20.0%) and nine (15.0%) of the patients, respectively. Dual resistance to clarithromycin and metronidazole was detected in five (8.3%) patients. Conclusion: These results indicate that the relatively high prevalence of the dual resistance in H. pylori isolates may need special attention and new therapeutic approaches in Japan.