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Clinical usefulness of edaravone for acute liver injury
Author(s) -
TADA SHINICHIRO,
NAKAMOTO NOBUHIRO,
KAMEYAMA KAORI,
TSUNEMATSU SATOSHI,
KUMAGAI NAOKI,
SAITO HIDETSUGU,
ISHII HIROMASA
Publication year - 2003
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2003.03064.x
Subject(s) - edaravone , carbon tetrachloride , medicine , free radical scavenger , bilirubin , lactate dehydrogenase , liver injury , olive oil , h&e stain , pharmacology , staining , endocrinology , oxidative stress , pathology , biochemistry , chemistry , enzyme , food science , organic chemistry
Background: Edaravone, a newly synthesized radical scavenger, has shown an excellent effect on treating stroke patients. The effect of edaravone on carbon tetrachloride (CCl 4 )‐induced acute liver injury was examined. Methods: Six rats were injected with CCl 4 alone and six rats were intravenously injected with edaravone immediately after and 3 h after injection of CCl 4 . Another six rats were injected with olive oil alone. The animals were killed at 24 h after the CCl 4 injection. Results: Injection of CCl 4 was followed by a marked increase in serum alanine aminotranferase (ALT) level (CCl 4 , 1630.6 ± 606.8 IU/L; olive oil, 21.0 ± 2.6 IU/L; P < 0.001), lactate dehydrogenase (LDH) level (CCl 4 , 5068.0 ± 2956.4 IU/L; olive oil, 203.6 ± 30.5 IU/L; P < 0.005), and total bilirubin (TB) level (CCl 4 , 0.88 ± 0.48 mg/dL; olive oil, 0.37 ± 0.05 mg/dL; P < 0.01), whereas in the edaravone‐treated rats, the ALT (119.4 ± 113.5 IU/L, P < 0.001), LDH (369.7 ± 288.2 IU/L, P < 0.005), and TB values (0.29 ± 0.16 mg/dL, P < 0.01) were significantly decreased. Histological examination of the liver by hematoxylin and eosin and oil red O staining showed a marked reduction of steatosis in the CCl 4 and edaravone‐treated rats compared with the CCl 4 ‐injected rats. Significant inhibition of hepatocytic apoptosis was demonstrated by the terminal deoxynucleotidyl transferase‐mediated UTP nick‐end labeling (TUNEL) method in the edaravone‐treated rats. Conclusions: These results suggest that edaravone has a marked preventive effect on oxidative stress‐induced acute liver injury. © 2003 Blackwell Publishing Asia Pty Ltd