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Relationship between human leukocyte antigen‐DRB1 and autoimmune hepatitis type I in Chinese patients
Author(s) -
QIU DEKAI,
MA XIONG
Publication year - 2003
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2003.02918.x
Subject(s) - medicine , human leukocyte antigen , autoimmune hepatitis , allele , immunology , hla drb1 , polymerase chain reaction , typing , autoimmune disease , hepatitis , antigen , relative risk , gene , gastroenterology , antibody , genetics , biology , confidence interval
Aim: To analyze the association of human leukocyte antigen (HLA)‐DRB1 with autoimmune hepatitis type I (AIH) among Chinese patients in the Shanghai area. Methods: In 32 patients and 48 healthy controls, polymerase chain reaction amplified with sequence‐specific primers (PCR‐SSP) was performed to elucidate the relevance of certain alleles or polymorphic sequences of HLA‐DRB1 with autoimmune hepatitis. Results: The HLA‐DRB1 typing by PCR‐SSP showed that DR4 had a significantly increased frequency among patients with AIH versus that of healthy controls (46.9 vs 20.8%; relative risk = 3.35, P = 0.014). In the subtypes of DR4, there was a trend of an increase in the gene frequency of DRB1*0405 in patients with AIH versus that of healthy controls (21.9 vs 6.3%, P = 0.04, but corrected P ( Pc ) = 0.08). In addition, our analysis indicated a significant increase in the alleles frequency encoding Leu‐Leu‐Glu‐Gln‐Lys‐Arg (LLEQRR) from the third hyperpolymorphic region (HVR3) of DR4 in the patients with AIH (86.7% of DR4 positive patients vs 40.0% in DR4 positive controls, P = 0.016, Pc = 0.028, relative risk (RR) = 9.75). Conclusion: Type I AIH among Chinese patients is associated with HLA‐DR4. There is a relevance of type I AIH and LLEQRR sequence within the third hyperpolymorphic region of the DRB1 allele. © 2003 Blackwell Publishing Asia Pty Ltd