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Interferon‐γ regulates apoptosis by releasing soluble tumor necrosis factor receptors in a gastric epithelial cell line
Author(s) -
FURUTA SHIMAKO,
GOTO HIDEMI,
NIWA YASUMASA,
OHMIYA NAOKI,
KAMIYA KENJI,
OGURI AKIHIKO,
HAYAKAWA TETSUO,
MORI NAOYOSHI
Publication year - 2002
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2002.02893.x
Subject(s) - tumor necrosis factor alpha , apoptosis , interferon , cell culture , cytotoxic t cell , medicine , cytokine , cancer research , interferon gamma , viability assay , receptor , immunology , cell , microbiology and biotechnology , biology , biochemistry , genetics , in vitro
Background and Aims: Interferon (IFN)‐γ and tumor necrosis factor (TNF) are predominant cytokines produced in the gastric mucosa of patients with Helicobacter pylori ‐infected gastritis. Several studies reported that IFN‐γ and TNF induced the synergistic effect on many cell lines. We attempted to clarify the apoptotic activity and the synergistic effect of IFN‐γ and TNF on the gastric epithelial cell, and whether IFN‐γ relates to soluble TNF receptors (sTNF‐R) release from the gastric epithelial cell.Methods: On the gastric epithelial cell line MKN45 , cytotoxic and apoptotic effects of IFN‐γ and TNF were examined. Next, sTNF‐R released in response to IFN‐γ and the protective effect of sTNF‐R against the cytotoxic activity of TNF and IFN‐γ were examined by blocking the release of sTNF‐R with a serine protease inhibitor such as phenylmethylsulfonyl fluoride.Results: Interferon‐γ significantly decreased cell viability, but TNF decreased it only slightly. Interferon‐γ and TNF did not make a synergistic effect on cell viability and apoptosis. Interferon‐γ and TNF induced sTNF‐R release from gastric epithelial cells. Phenylmethylsulfonyl fluoride significantly inhibited shedding of sTNF‐R and a synergistic effect of TNF and IFN‐γ on apoptosis was observed.Conclusion: These results suggest that sTNF‐R released by IFN‐γ regulate the injury on the gastric epithelial cell line induced by TNF. © 2002 Blackwell Publishing Asia Pty Ltd