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Relationship between nuclear factor‐κB activation and virulence factors of Helicobacter pylori in Japanese clinical isolates
Author(s) -
MAEDA SHIN,
ANARSANAA JAZAG,
MITSUNO YUZO,
HIRATA YOSHIHIRO,
AKANUMA MASAO,
IKENOUE TSUNEO,
OGURA KEIJI,
YOSHIDA HARUHIKO,
SHIRATORI YASUSHI,
OMATA MASAO
Publication year - 2002
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1046/j.1440-1746.2002.02738.x
Subject(s) - virulence , helicobacter pylori , virulence factor , polymerase chain reaction , gene , microbiology and biotechnology , medicine , pathogenesis , virology , biology , immunology , gastroenterology , genetics
Background:Several factors have been proposed as a possible virulence determinant of Helicobacter pylori infection. The aim of the present study was to evaluate these candidates in nuclear factor (NF)‐κΒ activation, which is a critical regulator of genes involved in inflammatory reactions.Methods:We determined the status of cagE , iceA , HP0441 (a virB4 homolog), the s1 signal sequence of vacA and babA2 by polymerase chain reaction, all of which are candidate virulence determinants, in 107 H. pylori strains isolated from Japanese patients. Nuclear factor‐κΒ activation was evaluated by the luciferase reporter assay. The gastric mucosa of the hosts was examined histologically.Results:The cagE gene was positive in 102 (95.3%) strains, iceA1 was positive in 71 (66.4%) strains, HP0441 was positive in 68 (63.6%) strains, vacA s1 was positive in 105 (98.1%) strains and babA2 was positive in 103 (96.3%) strains. Nuclear factor‐κΒ was activated by all cagE ‐positive strains, but was not activated by any of the cagE ‐negative strains. The status of iceA or HP0441 was not associated with NF‐κΒ activation. Neutrophil infiltration in gastric mucosa was significantly more severe in patients infected with cagE ‐positive strains than in patients infected with negative strains. No association was found between the degree of neutrophil infiltration and the status of HP0441 or iceA . Due to very high positivity of vacA s1 and babA2 in Japanese strains, their roles remain to be investigated.Conclusions:The cag pathogenicity island (PAI) status, as determined by cagE polymerase chain reaction, but not the status of iceA or HP0441 , is closely associated with NF‐κΒ activation and the degree of gastric mucosal inflammation in the hosts. © 2002 Blackwell Publishing Asia Pty Ltd